Nikon C2+ Confocal Microscope

Confocal microscopy is used for imaging of thick tissue sections.

Notable Features:

  • Four excitation lasers: 405nm, 488nm, 561nm, & 640nm, with outpout power >15mW each
  • 8 fps & 100 fps high-speed imaging
  • High performance optics: 10X/0.3, 20X/0.75,  & 60X/1.4 oil lenses

Raman Microscope

Raman microscopy uses light to excite molecules.

Notable Features:

  • 2.5 μm depth resolution. 785 nm high power NIR diode laser, suitable for biological samples
  • Motorized microscope stage for XY mapping with 1 nm step size
  • 785 nm laser with CCD detector with range from 400 nm to 1060 nm
  • Motorized beam expander for variable laser spot size from 1 μm to 300 μm

Location: FIU’s Engineering Center, room 2372.

For information regarding training and rates per use, please select the “Training” and “Rates” tabs. For more information, please contact the lab manager.

Scientifica Multiphoton Microscope

  • Custom Galvo-Galvo or Galvo-Resonant configuration
  • Scanning speeds up to 30Hz
  • Ti:sapphire laser with wavelengths 690-1040nm
  • Simultaneous dual channel fluorescence detection

 

Multiphoton Microscopy

  • Scientifica Multiphoton Microscope
  • Custom Galvo-Galvo or Galvo-Resonant configuration
  • Scanning speeds up to 30Hz
  • Ti:sapphire laser with wavelengths 690-1040nm
  • Simultaneous dual channel fluorescence detection
Rate (name)InternalExternal
Confocal Microscope$35/hour$51/hour

 

To check the core’s availability and schedule a time, please log into BME Core Facilities Booking system:

Make sure to have the proper core selected using the drop-down menu at the top of the page.

Select a time-slot under the piece of equipment you will be using to avoid double-scheduling. You will be instructed to log-in to finalize your appointment. Use your FIU credentials to log-in.

Required Training:

  • Read & sign the “Responsible Use” document
  • Read “Imaging Core Rules” document
  • Read User Manual
  • Watch tutorials (linked below)
  • Complete the following EH&S training & send certificates of completion to Lab Manager: Laboratory Hazard Awareness, Small Spills and Leaks, Hazard Communication (HAZCOM)

Resources:

Dr. Qian

yqian@fiu.edu

305-348-1409

Dr. Sally Duarte

anprasad@fiu.edu

305-348-6950