Histology Core
Leica CM1950 Cryostat
Leica CM1950 Cryostat has an enclosed microtome that yields high-quality sections, CryoZone system for optimal sectioning conditions, and easy-to-operate instrument controls.
Notable Features:
- Cryochamber temperature ranging from 0 to -35 degrees celsius
- Adjustable section thickness range from 0.5-100 micrometers
- Quick-freezing shelf for up to 15 specimens with temperature down to -42 degrees celsius
Flourescent Microscope
To be updated.
Location: FIU’s Modesto A. Maidique campus, Owa Ehan, room 312.
Information regarding training and rates per use, please select the “Training” and “Rates” tabs. For more information, please contact our lab manager.
| Rate (name) | Internal | External |
| Histology Core | $TBD/hour | $TBD/hour |
Our core facilities will provide most consumables for your use, free of charge. Each lab is responsible for purchasing their own chemicals/reagents/stains/antibodies. Any materials that are protocol-specific will need to be purchased by each respective lab.
Billing: Will be done on a monthly bases using FIU’s Smart Internal Billing. Lab Users please provide the lab manager with your PIs activity number that they use for funding.
Core operating hours: 8:30 am – 5 pm
To check the core’s availability and schedule a time, please log into BME Core Facilities Booking system:
Make sure to have the proper core selected using the drop-down menu at the top of the page. Select a time-slot under the piece of equipment you will be using.
Please Note: Scheduling for the following day must be done, at the latest, 24 hours in advance.
Lab Access: Access to the cores will be granted based on the alloted time of each individuals project. Once a project is completed, access will be terminated.
Required Training:
- Read & sign the “Responsible Use” document
- Read “Histology Core Rules” document
- Read Standard of Procedure document
- Complete the following EH&S training & send certificates of completion to Lab Manager: Laboratory Hazard Awareness, Small Spills and Leaks, Hazard Communication (HAZCOM)
Resources:
 | |
Alex Ricardo Paez 786-274-2961 | Dr. Sally Duarte |
Acute Slice Core
Leica VT1200 Vibrating-Blade Microtome
A Vibratome is a microtome with a vibrating blade. The vibration amplitude, speed, and blade angle can all be controlled.
Notable Features:
- Motorized movement of blade holder
- Adjustable section thickness from 1-1000 micrometers
- Memory for parameter settings for up to 8 individuals or techniques
Applied Biosystems QuantStudio 3 PCR System
A Real-time PCR system is used for specific detection and quantification of nucleic acid targets.
Notable Features:
- Fixed 96-well block (0.1 ml or 0.2 ml)
- 4-color multiplexing
- 3 VeriFlex temperature control zones
Sutter Pipette Puller:
A pipette puller is used to draw a single capillary glass tube evenly into two glass microelectrodes with symmetrical tips.
Notable Features:
- Safe heat mode to protect and extend filament life
- Built-in error detection of air pressure loss or filament burnout
- Access to previous pull results with the heat on times for each cycle of the program
Other Equipment: Centrifuge, 5L/10L Waterbath, Microplate Centrifuge, Incubating Mini-Shaker
Location: FIU’s Modesto A. Maidique campus, Academic Health Center 4, room 508.
Information regarding training and rates per use, please select the “Training” and “Rates” tabs. For more information, please contact our lab manager.
| Rate (name) | Internal | External |
| Acute Slice Core | $TBD/hour | $TBD/hour |
Our core facilities will provide most consumables for your use, free of charge. Each lab is responsible for purchasing their own chemicals/reagents/stains/antibodies. Any materials that are protocol-specific will need to be purchased by each respective lab.
Billing: Will be done on a monthly bases using FIU’s Smart Internal Billing. Lab Users please provide the lab manager with your PIs activity number that they use for funding.
To check the core’s availability and schedule a time, please log into BME Core Facilities Booking system:
Make sure to have the proper core selected using the drop-down menu at the top of the page. Select a time-slot under the piece of equipment you will be using.
Please Note: Scheduling for the following day must be done, at the latest, 24 hours in advance.
Lab Access: Access to the cores will be granted based on the alloted time of each individuals project. Once a project is completed, access will be terminated.
Required Training:
- Read & sign the “Responsible Use” document
- Read “Acute Slice Core Rules” document
- Read Standard of Procedures Document
- Complete the following EH&S training & send certificates of completion to Lab Manager: Laboratory Hazard Awareness, Small Spills and Leaks, Hazard Communication (HAZCOM)
Resources:
| |
Alex Ricardo Paez 786-274-2961 | Dr. Sally Duarte |